Iridian, the leader in optical filter solutions, now offers ultra performance filters specially designed for Real Time PCR (polymerase chain reaction) applications. Our standard filter solutions are compatible with common excitation sources and fluorophores but we specialize in customized filter solutions. The ultra PCR fluorescence filter sets are designed to provide a high signal-to-noise ratio of the probes contained in Real-Time PCR samples when illuminated with a LED light source.

Iridian’s emission, excitation and dichroic filters provide more signal with less background to the fluorescence instrument users and OEMs.

Dichroic filters

• Mechanical:
  – Filters: 12 x 8 mm, 12 x 17 mm
  – Filter Thickness 1.1 mm
  – Clear aperture: > 90%
  – Substrate: Fused Silica
  – Scratch/Dig: 60/40

• Optical:
  – Tx > 95% average on passbands
  – Reflection > 97% over blocking range
  – AOI: 45 +/- 2 degrees; CHA: 5 degrees
  – Steep slope: < 1% (90%T – 10%T)

Excitation and Emission Filters

• Mechanical:
  – Filters: 8 x 8 mm, 12 x 12 mm, 25 mm diam.
  – Filter Thickness 1.1 mm
  – Clear aperture: > 90%
  – Substrate: Fused Silica
  – Scratch/Dig: 60/40

• Optical:
  – Tx > 95% average on passbands
  – Average blocking > OD6 from 350-850 nm
  outside of passband
  – Steep slope: < 0.5% (50% Tmax- OD5)
  – AOI: 0 – 5 degrees; CHA: 5 degrees